Advances and perspectives in tissue clearing using CLARITY

Kristian H Reveles Jensen; Rune W Berg

doi:10.1016/j.jchemneu.2017.07.005

Advances and perspectives in tissue clearing using CLARITY

J Chem Neuroanat. 2017 Dec:86:19-34. doi: 10.1016/j.jchemneu.2017.07.005. Epub 2017 Jul 17.

Authors

Kristian H Reveles Jensen¹, Rune W Berg²

Affiliations

¹ University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark. Electronic address: krijen@sund.ku.dk.
² University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark. Electronic address: runeb@sund.ku.dk.

PMID: 28728966
DOI: 10.1016/j.jchemneu.2017.07.005

Abstract

CLARITY is a tissue clearing method, which enables immunostaining and imaging of large volumes for 3D-reconstruction. The method was initially time-consuming, expensive and relied on electrophoresis to remove lipids to make the tissue transparent. Since then several improvements and simplifications have emerged, such as passive clearing (PACT) and methods to improve tissue staining. Here, we review advances and compare current applications with the aim of highlighting needed improvements as well as aiding selection of the specific protocol for use in future investigations.

Keywords: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (PubChem CID: 15908); 2,2′-Thiodiethanol (PubChem CID: 5447); Acrylamide (PubChem CID: 6579); Biocytin; Brain; CLARITY; D-Sorbitol (PubChem CID: 5780); Diatrizoic acid (PubChem CID: 2140); Gylcerol (PubChem CID: 753); Histology; Immunohistochemistry; Iohexol (PubChem CID: 3730); Iomeprol-d3 (PubChem CID: 46781978); N,N,N’,N’-Tetrakis(2-hydroxypropyl)-ethylenediamine (amino alcohol, PubChem CID: 7615); Refractive index; Tissue clearing; Tracing; Virus; α-Thioglycerol (PubChem CID: 7291).

Publication types

Review

MeSH terms

Animals
Coloring Agents
Humans
Imaging, Three-Dimensional / methods*
Immunohistochemistry / methods*
Tissue Fixation / methods*

Substances

Coloring Agents