BSA-Sugar Conjugates as Ideal Building Blocks for SPR-Based Glycan Biosensors

Shun Tao; Tian-Wei Jia; Yang Yang; Li-Qiang Chu

doi:10.1021/acssensors.6b00679

BSA-Sugar Conjugates as Ideal Building Blocks for SPR-Based Glycan Biosensors

ACS Sens. 2017 Jan 27;2(1):57-60. doi: 10.1021/acssensors.6b00679. Epub 2017 Jan 3.

Authors

Shun Tao¹, Tian-Wei Jia¹, Yang Yang¹, Li-Qiang Chu¹

Affiliation

¹ College of Chemical Engineering and Materials Science and ‡China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Sino-French Joint Lab of Food Nutrition/Safety and Medicinal Chemistry, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science & Technology , No. 29, 13th Avenue, TEDA, Tianjin 300457, China.

PMID: 28722428
DOI: 10.1021/acssensors.6b00679

Abstract

Controlled immobilization of sugar probes is of key importance for the development of glycan biosensors. To this end, a series of BSA-sugar conjugates with different numbers of mannose units are prepared via the squaric acid-mediated coupling reaction. The conjugates can absorb directly on gold substrate without any derivation reactions, thus providing a simple and effective method for the construction of SPR-based glycan biosensors. SPR measurements show that the BSA-mannose conjugate with 11 mannoses exhibit the highest affinity to the lectin concanavalin A with a limit of detection of ca. 1.8 nM. Regeneration and specificity of the obtained glycan biosensors are also investigated.

Keywords: BSA-sugar conjugate; concanavalin A; glycan biosensor; mannose; surface plasmon resonance.