CRISPR/Cas9-Based Genome Editing in Plants

Yaling Zhang; Xingliang Ma; Xianrong Xie; Yao-Guang Liu

doi:10.1016/bs.pmbts.2017.03.008

CRISPR/Cas9-Based Genome Editing in Plants

Prog Mol Biol Transl Sci. 2017:149:133-150. doi: 10.1016/bs.pmbts.2017.03.008. Epub 2017 May 12.

Authors

Yaling Zhang¹, Xingliang Ma², Xianrong Xie¹, Yao-Guang Liu³

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou, China; College of Life Sciences, South China Agricultural University, Guangzhou, China.
² Global Institute for Food Security, University of Saskatchewan, Saskatoon, SK, Canada.
³ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou, China; College of Life Sciences, South China Agricultural University, Guangzhou, China. Electronic address: ygliu@scau.edu.cn.

PMID: 28712494
DOI: 10.1016/bs.pmbts.2017.03.008

Abstract

Recently, genome editing technologies have shown great potential in plants. The newly developed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a new generation of genome editing tool rapidly replacing the earlier zinc finger nucleases and transcription activator-like effector nucleases systems. Indeed, due to its advantages of simplicity and high efficiency, the CRISPR/Cas9-based genome editing system is becoming a powerful tool in plant science research. Here, we introduce the technical features of the plant CRISPR/Cas9-based genome editing system and its applications in plant functional genomics studies and genetic improvement.

Keywords: CRISPR/Cas9; DSDecode; Genome editing; Plant.

Publication types

Review

MeSH terms

CRISPR-Cas Systems / genetics*
Crops, Agricultural / genetics
Gene Editing*
Genome, Plant*
Mutation / genetics
Plants / genetics*