Virulent and pathogenic features on the Cronobacter sakazakii polymyxin resistant pmr mutant strain s-3

Xuerui Bao; Ling Yang; Lequn Chen; Bing Li; Lin Li; Yanyan Li; Zhenbo Xu

doi:10.1016/j.micpath.2017.07.022

Virulent and pathogenic features on the Cronobacter sakazakii polymyxin resistant pmr mutant strain s-3

Microb Pathog. 2017 Sep:110:359-364. doi: 10.1016/j.micpath.2017.07.022. Epub 2017 Jul 12.

Authors

Xuerui Bao¹, Ling Yang², Lequn Chen¹, Bing Li³, Lin Li³, Yanyan Li⁴, Zhenbo Xu⁵

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
² Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
³ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, China.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China. Electronic address: Yanyanli1123@hotmail.com.
⁵ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA. Electronic address: zhenbo.xu@hotmail.com.

PMID: 28711508
DOI: 10.1016/j.micpath.2017.07.022

Abstract

Cronobacter sakazakii is a well-known opportunistic pathogen responsible for necrotizing enterocolitis, meningitis and septicaemia in the premature, immunocompromised infants and neonates. This pathogen possesses various virulence factors and regulatory systems, and pmrA/pmrB regulatory system has been identified in a variety of bacterial species. The current study aims to investigate role of pmrA gene in the pathogenicity and virulence characteristics of Cronobacter sakazakii using whole genome sequencing and RNA-seq. Results demonstrated that the absence of pmrA has the potential to affect Cronobacter sakazakii on its pathogenicity, virulence and resistance abilities by regulating expression of numerous related genes, including CusB, CusC, CusR and ESA_pESA3p05434.

Keywords: Cronobacter sakazakii s-3; RNA-seq; Whole genome sequencing; pmrA gene.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Bacterial Proteins / physiology
Base Composition
Cronobacter sakazakii / genetics*
Cronobacter sakazakii / pathogenicity
DNA, Bacterial / isolation & purification
Drug Resistance, Bacterial / genetics*
Enterobacteriaceae Infections / microbiology
Gene Expression Regulation, Bacterial
Genome, Bacterial / genetics*
Polymyxins / pharmacology*
RNA, Bacterial / isolation & purification
Virulence
Virulence Factors / genetics*
Whole Genome Sequencing

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA, Bacterial
PhoQ protein, Bacteria
Polymyxins
RNA, Bacterial
Virulence Factors
pmrA protein, Bacteria