Rapid Detection of Apoptosis in Cultured Mammalian Cells

Igor Kudryavtsev; Maria Serebryakova; Liudmila Solovjeva; Maria Svetlova; Denis Firsanov

doi:10.1007/978-1-4939-7187-9_8

Rapid Detection of Apoptosis in Cultured Mammalian Cells

Methods Mol Biol. 2017:1644:105-111. doi: 10.1007/978-1-4939-7187-9_8.

Authors

Igor Kudryavtsev^{1

2}, Maria Serebryakova^{1

3}, Liudmila Solovjeva⁴, Maria Svetlova⁵, Denis Firsanov⁴

Affiliations

¹ Institute of Experimental Medicine, Saint-Petersburg, Russia.
² Far Eastern Federal University, Vladivostok, Russia.
³ State University of Information Technologies, Mechanics and Optics, Saint-Petersburg, Russia.
⁴ Institute of Cytology RAS, 4 Tikhoretski Avenue, Saint-Petersburg, Russia.
⁵ Institute of Cytology RAS, 4 Tikhoretski Avenue, Saint-Petersburg, Russia. svetlma@mail.ru.

PMID: 28710756
DOI: 10.1007/978-1-4939-7187-9_8

Abstract

Flow cytometry is a powerful tool for the analysis of apoptosis, the process that directly determines cell fate after the action of different stresses. Here, we describe a flow cytometry method for the assessment of early and late stages of apoptosis in non-fixed cultured cells using SYTO16, DRAQ7, and PO-PRO1 dyes simultaneously. This multicolor flow cytometry procedure requires 45 min for completion and provides a quantitative assessment of cell viability. It can be useful in evaluating the cytotoxic properties of new drugs, and antitumor interventions.

Keywords: Apoptosis; DRAQ7; Flow cytometry; Fluorescent nucleic acid dyes; PO-PRO-1; SYTO16.

MeSH terms

Apoptosis*
Flow Cytometry / methods*
Humans
Leukemia, Monocytic, Acute / pathology*
Tumor Cells, Cultured