Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331

Methods Mol Biol. 2017:1645:211-225. doi: 10.1007/978-1-4939-7183-1_15.

Abstract

The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc2155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step.

Keywords: ADD; Androstadienedione; Biotransformation; Mycobacterium smegmatis; Phytosterols; Steroids.

MeSH terms

  • Androstadienes / chemistry
  • Androstenedione / biosynthesis*
  • Androstenedione / chemistry
  • Biotechnology / methods*
  • Biotransformation*
  • Fermentation
  • Mycobacterium smegmatis / genetics
  • Mycobacterium smegmatis / metabolism
  • Phytosterols / chemistry*

Substances

  • Androstadienes
  • Phytosterols
  • 1,4-androstadiene-3,17-dione
  • Androstenedione