Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD⁺ on NiO Film Modified by GO and MBs

Jung-Chuan Chou; Siao-Jie Yan; Yi-Hung Liao; Chih-Hsien Lai; You-Xiang Wu; Cian-Yi Wu; Hsiang-Yi Chen; Hong-Yu Huang; Tong-Yu Wu

doi:10.3390/s17071618

Fabrication of Flexible Arrayed Lactate Biosensor Based on Immobilizing LDH-NAD⁺ on NiO Film Modified by GO and MBs

Sensors (Basel). 2017 Jul 12;17(7):1618. doi: 10.3390/s17071618.

Authors

Jung-Chuan Chou^{1

2}, Siao-Jie Yan³, Yi-Hung Liao⁴, Chih-Hsien Lai^{5

6}, You-Xiang Wu⁷, Cian-Yi Wu⁸, Hsiang-Yi Chen⁹, Hong-Yu Huang¹⁰, Tong-Yu Wu¹¹

Affiliations

¹ Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. choujc@yuntech.edu.tw.
² Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. choujc@yuntech.edu.tw.
³ Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. M10313322@yuntech.edu.tw.
⁴ Department of Information and Electronic Commerce Management, TransWorld University, Douliu 64063, Taiwan. liaoih@twu.edu.tw.
⁵ Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. chlai@yuntech.edu.tw.
⁶ Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. chlai@yuntech.edu.tw.
⁷ Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. M10513317@yuntech.edu.tw.
⁸ Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. M10513328@yuntech.edu.tw.
⁹ Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. B10213110@yuntech.edu.tw.
¹⁰ Department of Electronic Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. B10200017@yuntech.edu.tw.
¹¹ Graduate School of Mechanical Engineering, National Yunlin University of Science and Technology, Douliu 64002, Taiwan. D10011005@yuntech.edu.tw.

Abstract

We proposed the flexible arrayed lactate biosensor based on immobilizing l-lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide ( NAD + ) on nickel oxide (NiO) film, and which the average sensitivity could be enhanced by using graphene oxide (GO) and magnetic beads (MBs). By using GO and MBs, it exhibits excellent sensitivity (45.397 mV/mM) with a linearity of 0.992 in a range of 0.2 mM to 3 mM. According to the results of electrochemical impedance spectroscopy (EIS), the electron transfer resistance of LDH- NAD + -MBs/GPTS/GO/NiO film was smaller than those of LDH-NAD⁺/GPTS/GO/NiO film and LDH- NAD + /GPTS/NiO film, and it presented the outstanding electron transfer ability. After that, the limit of detection, anti-interference effect and bending test were also investigated.

Keywords: electrochemical impedance spectroscopy (EIS); graphene oxide (GO); lactate biosensor; magnetic beads (MBs); nickel oxide (NiO).

MeSH terms

Biosensing Techniques*
Graphite
L-Lactate Dehydrogenase
Lactic Acid
NAD

Substances

NAD
Lactic Acid
Graphite
L-Lactate Dehydrogenase