In vivo full-field functional optical hemocytometer

Fuli Zhang; Mingyi Wang; Dingan Han; Haishu Tan; Guojian Yang; Yaguang Zeng

doi:10.1002/jbio.201700039

In vivo full-field functional optical hemocytometer

J Biophotonics. 2018 Feb;11(2). doi: 10.1002/jbio.201700039. Epub 2017 Jul 31.

Authors

Fuli Zhang^{1

2}, Mingyi Wang¹, Dingan Han¹, Haishu Tan¹, Guojian Yang², Yaguang Zeng¹

Affiliations

¹ Department of Photoelectric Technology, Foshan University, Guangdong, China.
² Department of Physics and Applied Optics Beijing Area Major Laboratory, Beijing Normal University, Beijing, China.

PMID: 28700144
DOI: 10.1002/jbio.201700039

Abstract

We present an in vivo lab-free full-field functional optical hemocytometer (FFOH) for application to the capillaries of a live biological specimen, based on the absorption intensity fluctuation modulation (AIFM) effect. Because of the absorption difference between the red blood cells (RBCs) and background tissue under low-coherence light illumination, an endogenous instantaneous intensity fluctuation is generated by the AIFM effect when RBCs discontinuously traverse the capillary. The AIFM effect is used to highlight the RBC signal relative to the background tissue by computing the real-time modulation depth. FFOH can simultaneously provide a flow video, the flow velocity and the RBC count. Ourexperimental results can potentially be applied to study the physiological mechanisms of the blood circulation systems of near-transparent live biological samples.

Keywords: absorption; biomedical imaging; full-field; hemocytometer; in vivo; intensity fluctuation modulation; microcirculation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood Circulation
Capillaries / physiology
Erythrocyte Count / instrumentation*
Optical Devices*
Zebrafish / blood