Dopamine D1 receptors induce apoptosis of osteosarcoma cells via changes of MAPK pathway

Clin Exp Pharmacol Physiol. 2017 Nov;44(11):1166-1168. doi: 10.1111/1440-1681.12814.

Abstract

This study explored the effects and mechanisms of dopamine D1 receptors (DR1) activation on the apoptosis of osteosarcoma cells (OS732).The DR1 agonist SKF-38393 decreased the viability of OS732 cells and increased their rate of apoptosis, whereas the DR1 antagonist SCH-23390 abolished the effects of SKF-38393. In OS732 cells, overexpression of DR1 increased the rate of apoptosis, caspase-9 and -3 expression, and the release of cytochrome c (Cyt c), reduced Bcl-2 expression, inhibited extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation, and induced phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK). These results suggest that activation of DR1 induces osteosarcoma cell apoptosis via changes to the MAPK pathway.

Keywords: MAPK pathway; apoptosis; dopamine D1 receptors; osteosarcoma cells.

Publication types

  • Letter

MeSH terms

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / pharmacology
  • Apoptosis* / drug effects
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Humans
  • MAP Kinase Signaling System* / drug effects
  • Osteosarcoma / pathology*
  • Phosphorylation / drug effects
  • Receptors, Dopamine D1 / agonists
  • Receptors, Dopamine D1 / metabolism*

Substances

  • Receptors, Dopamine D1
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine