Spirosoma areae sp. nov., Isolated from Soil

Abstract

A yellow-colored Gram-negative strain, Arct 1-12^T, was isolated from a soil sample collected in Seoul Women's University, South Korea, and grown on R2A agar at 25 °C. Growth of strain Arct 1-12^T was observed at a temperature range of 15-30 °C (optimal 25 °C), but not at 4 or 42 °C. The strain tolerated up to 1% NaCl (w/v) and displayed optimal growth in the absence of NaCl. Growth occurred at pH 6.0-9.0 (optimally at pH 7). According to the 16S rRNA gene sequence, the strain is moderately related to Spirosoma spitsbergense DSM 19989^T (93.54%), S. endophyticum EX36^T (93.25%), S. linguale LMG 10896^T (92%), S. luteum DSM 19990^T (93.16%), S. panaciterrae DSM 21099^T (91.09%), S. oryzae RHs22^T (90.37%), and S. rigui WPCB118^T (91.54%). Chemotaxonomic analyses revealed that strain Arct 1-12^T possesses MK-7 as the predominant menaquinone, a polar lipid profile consisting of phosphatidylethanolamine, an unknown aminolipid, an unknown aminophospholipid, and an unknown lipid, and iso-C_15:0, C_16:1 ω5c and Summed Feature 3 (C_16:1 ω6c and/or C_16:1 ω7c) as the major fatty acids. The DNA G+C content is 52.3 mol %. Based on polyphasic evidence, strain Arct 1-12^T (=JCM 31025 ^T = KCTC 42814^T) is classified as the type strain of a novel Spirosoma species for which the name Spirosoma areae sp. nov. is proposed.