Two-dimensional ytterbium oxide nanodisks based biosensor for selective detection of urea

Ahmed A Ibrahim; Rafiq Ahmad; Ahmad Umar; M S Al-Assiri; A E Al-Salami; Rajesh Kumar; S G Ansari; S Baskoutas

doi:10.1016/j.bios.2017.06.015

Two-dimensional ytterbium oxide nanodisks based biosensor for selective detection of urea

Biosens Bioelectron. 2017 Dec 15:98:254-260. doi: 10.1016/j.bios.2017.06.015. Epub 2017 Jun 15.

Authors

Ahmed A Ibrahim¹, Rafiq Ahmad², Ahmad Umar³, M S Al-Assiri⁴, A E Al-Salami⁵, Rajesh Kumar⁶, S G Ansari⁷, S Baskoutas⁸

Affiliations

¹ Department of Chemistry, Faculty of Science and Arts, Najran University, P.O. Box 1988, Najran 11001, Saudi Arabia; Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, P.O.Box-1988, Najran 11001, Saudi Arabia; Department of Materials Science, University of Patras, Patras GR-26504, Greece.
² School of Semiconductor and Chemical Engineering, Nanomaterials Processing Research Center, Chonbuk National University, 567 Baekjedaero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
³ Department of Chemistry, Faculty of Science and Arts, Najran University, P.O. Box 1988, Najran 11001, Saudi Arabia; Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, P.O.Box-1988, Najran 11001, Saudi Arabia. Electronic address: ahmadumar786@gmail.com.
⁴ Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, P.O.Box-1988, Najran 11001, Saudi Arabia; Department of Physics, Faculty of Science and Arts, Najran University, P.O. Box 1988, Najran 11001, Saudi Arabia.
⁵ Department of Physics, Faculty of Science, King Khalid University, P.O.Box-9004, Abha 61413, Saudi Arabia.
⁶ PG Department of Chemistry, JCDAV College, Dasuya 144205, Punjab, India.
⁷ Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
⁸ Department of Materials Science, University of Patras, Patras GR-26504, Greece.

PMID: 28689111
DOI: 10.1016/j.bios.2017.06.015

Abstract

Herein, we demonstrate synthesis and application of two-dimensional (2D) rectangular ytterbium oxide (Yb₂O₃) nanodisks via a facile hydrothermal method. The structural, morphological, compositional, crystallinity, and phase properties of as-synthesized nanodisks were carried out using several analytical techniques that showed well defined 2D rectangular nanodisks/sheet like morphologies. The average thickness and edge length of the nanosheet structures were 20 ± 5nm and 600 ± 50nm, respectively. To develop urea biosensor, glassy carbon electrodes (GCE) were modified with Yb₂O₃ nanodisks, followed by urease immobilization and Nafion membrane covering (GCE/Yb₂O₃/Urease/Nafion). The fabricated biosensor showed sensitivity of 124.84μAmM^-1cm^-2, wide linear range of 0.05-19mM, detection limit down to ~ 2μM, and fast response time of ~ 3s. The developed biosensor was also used for the urea detection in water samples through spike-recovery experiments, which illustrates satisfactory recoveries. In addition, the obtained desirable selectivity towards specific interfering species, long-term stability, reproducibility, and repeatability further confirm the potency of as-fabricated urea biosensor.

Keywords: Urea biosensor; Urease; Ytterbium oxide nanodisks.

MeSH terms

Biosensing Techniques*
Electrodes
Enzymes, Immobilized / chemistry
Nanostructures / chemistry*
Oxides / chemistry
Urea / chemistry
Urea / isolation & purification*
Urease / chemistry
Ytterbium / chemistry*

Substances

Enzymes, Immobilized
Oxides
Urea
Urease
Ytterbium