Importance of the surface chemistry of nanoparticles on peroxidase-like activity

Ramy El-Sayed; Fei Ye; Heba Asem; Radwa Ashour; Wenyi Zheng; Mamoun Muhammed; Moustapha Hassan

doi:10.1016/j.bbrc.2017.07.014

Importance of the surface chemistry of nanoparticles on peroxidase-like activity

Biochem Biophys Res Commun. 2017 Sep 9;491(1):15-18. doi: 10.1016/j.bbrc.2017.07.014. Epub 2017 Jul 4.

Authors

Ramy El-Sayed¹, Fei Ye², Heba Asem³, Radwa Ashour⁴, Wenyi Zheng⁵, Mamoun Muhammed⁶, Moustapha Hassan⁷

Affiliations

¹ Division of Experimental Cancer Medicine (ECM), Department of Laboratory Medicine, Karolinska Institutet, SE-141 86 Stockholm, Sweden. Electronic address: ramy.el.sayed@ki.se.
² Division of Experimental Cancer Medicine (ECM), Department of Laboratory Medicine, Karolinska Institutet, SE-141 86 Stockholm, Sweden. Electronic address: feiy@kth.se.
³ Division of Functional Materials, KTH Royal Institute of Technology, SE-164 40 Stockholm, Sweden. Electronic address: hebaasem@kth.se.
⁴ Division of Functional Materials, KTH Royal Institute of Technology, SE-164 40 Stockholm, Sweden. Electronic address: radwa.ashour@kth.se.
⁵ Division of Experimental Cancer Medicine (ECM), Department of Laboratory Medicine, Karolinska Institutet, SE-141 86 Stockholm, Sweden. Electronic address: wenyi.zheng@ki.se.
⁶ Division of Functional Materials, KTH Royal Institute of Technology, SE-164 40 Stockholm, Sweden; Institute of Graduate Studies & Research, Alexandria University, Alexandria, Egypt. Electronic address: mamoun@kth.se.
⁷ Division of Experimental Cancer Medicine (ECM), Department of Laboratory Medicine, Karolinska Institutet, SE-141 86 Stockholm, Sweden; Clinical Research Center, Novum, Karolinska University Hospital-Huddinge, SE-141 86 Stockholm, Sweden. Electronic address: moustapha.hassan@ki.se.

PMID: 28687493
DOI: 10.1016/j.bbrc.2017.07.014

Abstract

We report the studies on origin of peroxidase-like activity for gold nanoparticles, as well as the impact from morphology and surface charge of nanoparticles. For this purpose, we have synthesized hollow gold nanospheres (HAuNS) and gold nanorods (AuNR) with different morphology and surface chemistry to investigate their influence on the catalytic activity. We found that citrate-capped HAuNS show catalyzing efficiency in oxidation reaction of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide (H₂O₂) and it is superior to that of cetyltrimethylammonium bromide (CTAB)-capped AuNR. The kinetics of catalytic activities from HAuNS and AuNR were respectively studied under varied temperatures. The results indicated that surface chemistry rather than morphology of nanoparticles plays an important role in the catalytic reaction of substrate. Furthermore, influencing factors such as pH, amount of nanoparticle and H₂O₂ concentration were also investigated on HAuNS-catalyzed system. The great impact of nanoparticle surface properties on catalytic reactions makes a paradigm in constructing nanozymes as peroxidase mimic for sensing application.

Keywords: Enzyme mimic; Gold nanorods; Hollow gold nanospheres; Peroxidase activity; Surface chemistry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Benzidines / chemistry*
Enzyme Activation
Gold / chemistry*
Hydrogen Peroxide / chemistry*
Materials Testing
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Oxidation-Reduction
Particle Size
Peroxidase / chemistry*
Peroxidase / ultrastructure
Substrate Specificity
Surface Properties

Substances

Benzidines
3,3',5,5'-tetramethylbenzidine
Gold
Hydrogen Peroxide
Peroxidase