AuNP-CTG based probing system targeting CAG repeat DNA and RNA sequences

Binh Huy Le; Han Na Joo; Do Won Hwang; Kyu Wan Kim; Young Jun Seo

doi:10.1016/j.bmcl.2017.06.067

AuNP-CTG based probing system targeting CAG repeat DNA and RNA sequences

Bioorg Med Chem Lett. 2017 Aug 15;27(16):3772-3775. doi: 10.1016/j.bmcl.2017.06.067. Epub 2017 Jun 27.

Authors

Binh Huy Le¹, Han Na Joo², Do Won Hwang³, Kyu Wan Kim⁴, Young Jun Seo⁵

Affiliations

¹ Department of Bioactive Material Sciences, Chonbuk National University, South Korea.
² Department of Chemistry, Chonbuk National University, South Korea.
³ Department of Nuclear Medicine, Seoul National University College of Medicine, South Korea; Medical Research Center, Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, South Korea.
⁴ Medical Research Center, Institute of Radiation Medicine, Seoul National University College of Medicine, Seoul, South Korea.
⁵ Department of Bioactive Material Sciences, Chonbuk National University, South Korea; Department of Chemistry, Chonbuk National University, South Korea. Electronic address: yseo@jbnu.ac.kr.

PMID: 28687202
DOI: 10.1016/j.bmcl.2017.06.067

Abstract

We have developed a AuNP-CTG based probing system that is applicable to the detection of many units of CAG repeat sequences which was synthesized by a rolling circle amplification (RCA) system with changes in fluorescence. We also demonstrate that our AuNP-CTG based probing system could transfect without using transfection reagent and detect target CAG repeat sequences in HeLa cells with dramatic changes in fluorescence. This AuNP-CTG based probing system could also be used, in conjunction with the CAG repeat RCA system, to detect target DNA. This system was so sensitive to the target DNA that it could detect even picomolar amounts with amplification of the fluorescence signal. Furthermore, we have used our gold-based CAG probing system for the detection of RNA CAG repeat sequences.

Keywords: CNG repeat sequence; Fluorescence; Gold nanoparticles; In vivo imaging; Rolling circle amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / chemistry*
DNA Probes / chemistry*
Gold / chemistry*
HeLa Cells
Humans
Nanoparticles / chemistry*
RNA / chemistry*
Trinucleotide Repeats

Substances

DNA Probes
RNA
Gold
DNA