Identification and characterization of Burkholderia multivorans CCA53

Hironaga Akita; Zen-Ichiro Kimura; Mohd Zulkhairi Mohd Yusoff; Nobutaka Nakashima; Tamotsu Hoshino

doi:10.1186/s13104-017-2565-1

Identification and characterization of Burkholderia multivorans CCA53

BMC Res Notes. 2017 Jul 6;10(1):249. doi: 10.1186/s13104-017-2565-1.

Authors

Hironaga Akita¹, Zen-Ichiro Kimura², Mohd Zulkhairi Mohd Yusoff^{3

4}, Nobutaka Nakashima^{5

6}, Tamotsu Hoshino^{3

5}

Affiliations

¹ Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan. h-akita@aist.go.jp.
² Department of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Aga-minami, Kure, Hiroshima, 737-8506, Japan.
³ Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan.
⁴ Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
⁵ Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, Hokkaido, 062-8517, Japan.
⁶ Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 2-12-1-M6-5 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan.

Abstract

Objective: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53.

Results: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247^T. The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53.

Keywords: Burkholderia multivorans; Lignin-degrading bacterium; MLST analysis; Second-generation biofuel.

MeSH terms

Biofuels*
Burkholderia*
Multilocus Sequence Typing / methods*
Soil Microbiology*

Substances

Biofuels