Generation of human induced pluripotent stem cells from urinary cells of a healthy donor using a non-integration system

Kyung-Ok Uhm; Eun Hee Jo; Gue Youn Go; So-Jung Kim; Hye Young Choi; Young Sam Im; Hye-Yeong Ha; Ji-Won Jung; Soo Kyung Koo

doi:10.1016/j.scr.2017.03.019

Generation of human induced pluripotent stem cells from urinary cells of a healthy donor using a non-integration system

Stem Cell Res. 2017 May:21:44-46. doi: 10.1016/j.scr.2017.03.019. Epub 2017 Mar 30.

Authors

Kyung-Ok Uhm¹, Eun Hee Jo¹, Gue Youn Go¹, So-Jung Kim¹, Hye Young Choi¹, Young Sam Im¹, Hye-Yeong Ha¹, Ji-Won Jung¹, Soo Kyung Koo²

Affiliations

¹ Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
² Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea. Electronic address: skkoo@nih.go.kr.

PMID: 28677537
DOI: 10.1016/j.scr.2017.03.019

Abstract

Urinary cells can be an ideal source for generating hiPSCs and progenitors, as they are easily accessible, non-invasive, and universally available. We generated human induced pluripotent stem cells (hiPSCs) from the urinary cells of a healthy donor using a Sendai virus-based gene delivery method. The generated hiPSC line, KSCBi001-A, has a normal karyotype (46,XY). The pluripotency and capacity of multilineage differentiation were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from National Stem Cell Bank, Korea National Institute of Health.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Male
Sendai virus*
Transduction, Genetic*
Urine / cytology*