Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a non-integration system

Kyung-Ok Uhm; So-Jung Kim; Eun Hee Jo; Gue Youn Go; Hye Young Choi; Young Sam Im; Hye-Yeong Ha; Jung-Hyun Kim; Soo Kyung Koo

doi:10.1016/j.scr.2017.03.009

Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a non-integration system

Stem Cell Res. 2017 May:21:13-15. doi: 10.1016/j.scr.2017.03.009. Epub 2017 Mar 21.

Authors

Kyung-Ok Uhm¹, So-Jung Kim¹, Eun Hee Jo¹, Gue Youn Go¹, Hye Young Choi¹, Young Sam Im¹, Hye-Yeong Ha¹, Jung-Hyun Kim¹, Soo Kyung Koo²

Affiliations

¹ Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, Republic of Korea.
² Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, Republic of Korea. Electronic address: skkoo@nih.go.kr.

PMID: 28677527
DOI: 10.1016/j.scr.2017.03.009

Abstract

We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts using a Sendai virus (SeV)-based gene delivery method. The generated hiPSC line, KSCBi002-A, has a normal karyotype (46,XY). The pluripotency and differentiation capacity were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from the National Stem Cell Bank, Korea National Institute of Health.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Dermis / cytology
Dermis / metabolism*
Fibroblasts / cytology
Fibroblasts / metabolism*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Male
Sendai virus*
Transduction, Genetic*