Ultrafine particles (UFPs) from domestic wood stoves: genotoxicity in human lung carcinoma A549 cells

Mutat Res Genet Toxicol Environ Mutagen. 2017 Aug:820:39-46. doi: 10.1016/j.mrgentox.2017.06.001. Epub 2017 Jun 16.

Abstract

In this paper, results on the potential toxicity of ultrafine particles (UFPs d<100nm) emitted by the combustion of logwood and pellet (hardwood and softwood) are reported. The data were collected during the TOBICUP (TOxicity of BIomass COmbustion generated Ultrafine Particles) project, carried out by a team composed of interdisciplinary research groups. The genotoxic evaluation was performed on A549 cells (human lung carcinomacells) using UFPs whose chemical composition was assessed by a suite of analytical techniques. Comet assay and γ-H2AX evaluation show a significant DNA damage after 24h treatment. The interpretation of the results is based on the correlation among toxicological results, chemical-physical properties of UFPs, and the type and efficiency conditions in residential pellet or logwood stoves.

MeSH terms

  • A549 Cells
  • Air Pollution, Indoor / adverse effects
  • Cell Survival / drug effects
  • Comet Assay
  • Cooking
  • DNA Damage*
  • Fires*
  • Histones / genetics
  • Humans
  • Mutagens / analysis
  • Mutagens / chemistry
  • Mutagens / toxicity*
  • Nanoparticles / analysis
  • Nanoparticles / chemistry
  • Nanoparticles / toxicity*
  • Particle Size
  • Particulate Matter / analysis
  • Particulate Matter / chemistry
  • Particulate Matter / toxicity*
  • Reactive Oxygen Species / metabolism
  • Surface Properties
  • Wood / chemistry*

Substances

  • H2AX protein, human
  • Histones
  • Mutagens
  • Particulate Matter
  • Reactive Oxygen Species