Objective: To investigate the inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the proliferation of salivary adenoid cystic carcinoma ACC-83 cells.
Methods: In vitro cell culture was performed. The proliferation of ACC-83 cells treated with different concentrations (5, 10, 20, 40, 60, 80, 100 μg·mL⁻¹) of total saponins from Paris polyphylla var. yunnanensis was observed using CCK-8 assay. Meanwhile, the apoptosis of ACC-83 cells treated with different concentrations (25, 50, 100 μg·mL⁻¹) of the total saponins was observed using flow cytometry. The expression levels of macrophage migration inhibitory factor (MIF) and CD74 were measured using Western blot and reverse transcription-polymerase chain reaction.
Results: The total saponins from Paris polyphylla var. yunnanensis induced apoptosis and expressed dose-effect relationship. ACC-83 cells expressed MIF and CD74, and the total saponins suppressed MIF and CD74 expression in ACC-83 cells.
Conclusions: The total saponins from Paris polyphylla var. yunnanensis can significantly inhibit the proliferation, suppress MIF and CD74 expression, and promote apoptosis in ACC-83 cells. This study provides a theoretical basis for the treatment of salivary adenoid cystic carcinoma using Paris polyphylla var. yunnanensis. .
目的 探讨滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞的增殖抑制作用及其相关机制，为滇重楼治疗涎腺腺样囊性癌提供理论依据。方法 通过体外细胞培养，采用CCK-8法检测不同药物质量浓度（5、10、20、40、60、80、100 μg·mL⁻¹）的滇重楼总皂苷对ACC-83细胞的增殖抑制效应，采用流式细胞仪检测不同药物质量浓度（25、50、100 μg·mL⁻¹）的滇重楼总皂苷对ACC-83细胞的凋亡率，Western blot及逆转录聚合酶链反应法检测巨噬细胞游走抑制因子（MIF）和CD74的表达。结果 滇重楼总皂苷可促进ACC-83细胞凋亡，并呈剂量-效应关系。ACC-83细胞中存在MIF和CD74的表达，滇重楼总皂苷可抑制MIF和CD74的表达。结论 滇重楼总皂苷对ACC-83细胞的生长有明显抑制作用，可以抑制MIF及CD74的表达，诱导细胞凋亡，为其临床应用提供了依据。.
Keywords: macrophage migration inhibitory factor; salivary adenoid cystic carcinoma; total saponins from Paris polyphylla var. yunnanensis.