Synthesis of the exopolysaccharide-based bioflocculant Rhizobium radiobacter SZ4S7S14 was researched and medium optimized for enhanced production of bioflocculant. D-Mannose and yeast extract were found to be best carbon and nitrogen sources for maximal yield of bioflocculant. The bioflocculant samples obtained in different media with different carbon and nitrogen sources were further analyzed by SEM-EDX and FT-IR. FT-IR spectroscopy of the bioflocculant samples, obtained in different carbon/nitrogen sources showed slight modification of the structures of biopolymers. SEM micrographs revealed that exopolysaccharide-based bioflocculant of R. radiobacter SZ4S7S14 looks like bricks, and chemical structure of it can be varied due to utilized carbon and nitrogen source.
Keywords: Exopolysaccharide-based bioflocculant; Optimization; Rhizobium radiobacter; Synthesis.