Fiber bundle-based integrated platform for wide-field fluorescence imaging and patterned optical stimulation for modulation of vasoconstriction in the deep brain of a living animal

Minkyung Kim; Jinki Hong; Jinsik Kim; Hyun-Joon Shin

doi:10.1364/BOE.8.002781

Fiber bundle-based integrated platform for wide-field fluorescence imaging and patterned optical stimulation for modulation of vasoconstriction in the deep brain of a living animal

Biomed Opt Express. 2017 May 1;8(6):2781-2795. doi: 10.1364/BOE.8.002781. eCollection 2017 Jun 1.

Authors

Minkyung Kim^{1

2}, Jinki Hong^{1

2}, Jinsik Kim³, Hyun-Joon Shin^{1

2}

Affiliations

¹ Center for Bionics, Korea Institute of Science and Technology (KIST), Seoul, South Korea.
² Biomedical Engineering, Korea University of Science and Technology, Daejeon, South Korea.
³ Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul, South Korea.

Abstract

We report a fiber optics-based intravital fluorescence imaging platform that includes epi-fluorescence microscopy and laser patterned-light stimulation system. The platform can perform real-time fluorescence imaging with a lateral resolution of ~4.9 μm while directly inserted into the intact mouse brain, optically stimulate vasoconstriction during real-time imaging, and avoid vessel damage in the penetration path of imaging probe. Using 473-nm patterned-light stimulation, we successfully modulated the vasoconstriction of a single targeted 37-μm-diameter blood vessel located more than 4.7 mm below the brain surface of a live SM22-ChR2 mouse. This platform may permit the hemodynamic studies associated with deeper brain neurovascular disorders.

Keywords: (110.2350) Fiber optics imaging; (130.0130) Integrated optics; (170.1470) Blood or tissue constituent monitoring; (170.2520) Fluorescence microscopy.