α, ω-Dodecanedioic acid production by Candida viswanathii ipe-1 with co-utilization of wheat straw hydrolysates and n-dodecane

Abstract

Candida viswanathii ipe-1 was used to produce α, ω-dodecanedioic acid (DC₁₂), which showed capability to ferment xylose and glucose simultaneously, while arabinose utilization was less efficient. A low concentration of furfural enhanced cell growth, and the addition of 4.0g/L sodium acetate largely increased DC₁₂ production. It indicated that detoxification of the wheat straw hydrolysates was not necessary for the biosynthesis of DC₁₂. Based on the promising features of our strain, an efficient process was developed to produce DC₁₂ from co-utilization of wheat straw hydrolysates and n-dodecane. Using this process, 129.7g/L DC₁₂ with a corresponding productivity of 1.13g·L^-1·h^-1 could be produced, which was increased by 40.0% compared with a sole carbon of glucose. The improved DC₁₂ yield by the co-utilization of wheat straw hydrolysates and n-dodecane using C. viswanathii ipe-1 demonstrates the great potential of using biomass as a feedstock in the production of DC₁₂.

Keywords: Candida viswanathii; Wheat straw; n-Dodecane; α, ω-Dodecanedioic acid.