High-performance thin-layer chromatography (HPTLC) is a very robust, fast, and inexpensive technique that enables separation of complex mixtures. Here, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analyses for structural determination.
Keywords: Cerebrosides; Galactosylceramide; Glucosylceramide; High-performance thin-layer chromatography; Orcinol reagent.