We describe here a step-by-step protocol for extraction of lipids from tissue/cell samples for biomedical research. The protocol described here works well for biological samples that contain lipids, which are less than 2% of weight compared to total wet weight of tissue or cells. The protocols described here are suitable and incremental modification of previously published protocols. These protocols have been developed based on our experience with different tissues and cells, and yield estimates determined using class-specific mass spectrometry either using direct infusion or after chromatographic separation.
Keywords: Bligh and Dyer method; Bradford assay; Lipid extraction; Lipid sample; Protein quantification.