Fine Needle Aspiration Combined With Matrix-assisted Laser Desorption Ionization Time-of-Flight/Mass Spectrometry to Characterize Lipid Biomarkers for Diagnosing Accuracy of Breast Cancer

Yi-Tzu Cho; Hung Su; Yi-Yan Chiang; Jentaie Shiea; Shyng-Shiou F Yuan; Wen-Chun Hung; Yao-Tsung Yeh; Ming-Feng Hou

doi:10.1016/j.clbc.2017.04.014

Fine Needle Aspiration Combined With Matrix-assisted Laser Desorption Ionization Time-of-Flight/Mass Spectrometry to Characterize Lipid Biomarkers for Diagnosing Accuracy of Breast Cancer

Clin Breast Cancer. 2017 Aug;17(5):373-381.e1. doi: 10.1016/j.clbc.2017.04.014. Epub 2017 May 25.

Authors

Yi-Tzu Cho¹, Hung Su², Yi-Yan Chiang², Jentaie Shiea³, Shyng-Shiou F Yuan⁴, Wen-Chun Hung⁵, Yao-Tsung Yeh⁶, Ming-Feng Hou⁷

Affiliations

¹ Department of Cosmetic Applications and Management, Yuh-Ing Junior College of Health Care and Management, Kaohsiung, Taiwan.
² Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
³ Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: jetea@mail.nsysu.edu.tw.
⁴ Translational Research Center and Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁵ National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan.
⁶ Department of Medical Laboratory Sciences and Biotechnology, Fooyin University, Kaohsiung, Taiwan.
⁷ Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Kaohsiung Municipal Siaogang Hospital, Kaohsiung, Taiwan.

PMID: 28648841
DOI: 10.1016/j.clbc.2017.04.014

Abstract

Background: Fine needle aspiration (FNA) cytology has been widely used for pathologic assessment of breast lesions. However, the examination suffers a risk of false-negative results owing to insufficient sample volumes, inaccurate sampling positions, nondefinitive cytologic features, or suboptimal cell preservation. One approach to improve its accuracy is using modern mass spectrometry to detect disease biomarkers, of which the tissue samples are collected through FNA.

Methods: The biological compounds in the FNA tissue samples were extracted and characterized by matrix-assisted laser desorption ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). The results were further analyzed by principal component analysis. Distribution of lipid biomarkers on tissues was explored by imaging mass spectrometry.

Results: Lipid profiles of the tissue samples collected by FNA were rapidly obtained through MALDI-TOF/MS analysis. Phosphatidylcholines and triacylglycerols were detected as the predominant compounds in cancerous and normal regions, respectively. The samples were clearly classified by principal component analysis, based on the differences in their lipid profiles. Different lipid patterns were clearly viewed through the molecular imaging of normal and tumorous regions of breast tissue samples.

Conclusion: The FNA-MALDI-TOF/MS approach can provide complementary information for pathological examinations and improve the accuracy of breast cancer diagnoses. Owing to the ease of operation and automation, it is possible to efficiently screen the lipid biomarkers in a large number of tissue samples by means of MALDI-TOF/MS.

Keywords: FNA; Imaging mass spectrometry; Lipids; MALDI-TOF; PCA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor / metabolism*
Biopsy, Fine-Needle
Breast Neoplasms / diagnosis*
Breast Neoplasms / metabolism
Carcinoma, Ductal, Breast / diagnosis*
Carcinoma, Ductal, Breast / metabolism
Carcinoma, Intraductal, Noninfiltrating / diagnosis*
Carcinoma, Intraductal, Noninfiltrating / metabolism
Female
Follow-Up Studies
Humans
Lipids / analysis*
Neoplasm Invasiveness
Prognosis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Biomarkers, Tumor
Lipids