Establishment of a primary culture of polymorphous low grade adenocarcinoma cells

Abstract

Objective: The aim of the present study was to establish a primary cell culture derived from polymorphous low grade adenocarcinoma (PLGA).

Design: The neoplastic cells were derived from a 57-year-old female patient diagnosed with PLGA. A fragment of the tumor was collected and submitted to enzymatic digestion followed by centrifugation on a Percoll gradient. The cell population was characterized by means of immunofluorescence and detection of PRKD1 gene mutations.

Results: Epifluorescence analysis of the primary culture revealed that the malignant epithelial cells were predominantly polygonal in shape and positive for cytokeratin 7, vimentin, and S100. The doubling time of the cell culture was 86.73h. The restriction digestion assay showed that the neoplastic cells possess PRKD1 gene mutations.

Conclusion: The establishment of primary cell culture derived from PLGA should be considered a useful tool for molecular analysis of this salivary gland tumor.

Keywords: Adenocarcinoma; Cell culture techniques; Salivary gland neoplasms.