Activity of Tagetes minuta Linnaeus (Asteraceae) essential oil against L3 Anisakis larvae type 1

Filippo Giarratana; Daniele Muscolino; Graziella Ziino; Alessandro Giuffrida; Stefania Maria Marotta; Vittorio Lo Presti; Vincenzo Chiofalo; Antonio Panebianco

doi:10.1016/j.apjtm.2017.05.005

Activity of Tagetes minuta Linnaeus (Asteraceae) essential oil against L3 Anisakis larvae type 1

Asian Pac J Trop Med. 2017 May;10(5):461-465. doi: 10.1016/j.apjtm.2017.05.005. Epub 2017 May 17.

Authors

Filippo Giarratana¹, Daniele Muscolino¹, Graziella Ziino², Alessandro Giuffrida¹, Stefania Maria Marotta¹, Vittorio Lo Presti¹, Vincenzo Chiofalo¹, Antonio Panebianco¹

Affiliations

¹ Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy.
² Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy. Electronic address: gziino@unime.it.

PMID: 28647183
DOI: 10.1016/j.apjtm.2017.05.005

Abstract

Objective: To evaluate in vitro effects of Tagetes minuta L. essential oil (TEO) on L3 Anisakis larvae type 1.

Methods: In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae three different media were tested: 1) a saline solution (SS); 2) an industrial marinating solution (MS); 3) sunflower seeds oil (SO). For each media and concentrations of TEO (0.1%, 0.5%, 1.0% and 5.0% v/v), 20 parasites were introduced into plastic Petri dishes (diameter 90 mm) and maintained at room temperature. As controls, larvae were maintained without TEO under identical experimental conditions in SS, MS and SO. A total of 900 larvae were tested. The normalized mean viability, LT100, LT50 and the percentage of inactivation at 24 h were calculated.

Results: In vitro tests revealed a complete inactivation of parasites in saline solution after 2 h with 5% and 1% of TEO. In marinating solution, a complete inactivation of parasites was observed after 4 h at all concentrations used. A slower activity for all TEO concentration was reported in SO.

Conclusions: The results obtained, showing a strong activity against Anisakis larvae, confirm TEO as a larvicidal agent in the treatment of human anisakidosis and in the industrial marinating process.

Keywords: Anisakis; Biocidal activity; Essential oil; Parasites; Tagetes minuta.