Genome Editing of Monkey

Zhen Liu; Yijun Cai; Qiang Sun

doi:10.1007/978-1-4939-7128-2_12

Genome Editing of Monkey

Methods Mol Biol. 2017:1630:141-152. doi: 10.1007/978-1-4939-7128-2_12.

Authors

Zhen Liu¹, Yijun Cai¹, Qiang Sun^{2

3}

Affiliations

¹ Nonhuman Primate Research (SuZhou) Facility, Institute of Neuroscience, Chinese Academy of Sciences (CAS) Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, CAS, Shanghai, 200031, China.
² Nonhuman Primate Research (SuZhou) Facility, Institute of Neuroscience, Chinese Academy of Sciences (CAS) Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, CAS, Shanghai, 200031, China. qsun@ion.ac.cn.
³ Nonhuman Primate Research (SuZhou) Facility, Institute of Neuroscience, Chinese Academy of Sciences, Room A2304, Biological Research Building, 320 Yue Yang Road, Shanghai, 200031, China. qsun@ion.ac.cn.

PMID: 28643256
DOI: 10.1007/978-1-4939-7128-2_12

Abstract

Gene-modified monkey models would be particularly valuable in biomedical and neuroscience research. Virus-based transgenic and programmable nucleases-based site-specific gene editing methods (TALEN, CRISPR-cas9) enable the generation of gene-modified monkeys with gain or loss of function of specific genes. Here, we describe the generation of transgenic and knock-out (KO) monkeys with high efficiency by lentivirus and programmable nucleases.

Keywords: Gene-modified monkeys; Knockout; Lentivirus; Programmable nucleases; Transgenic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified
CRISPR-Cas Systems
Endonucleases / metabolism
Female
Gene Editing / methods*
Gene Editing / veterinary
Gene Knockout Techniques
Haplorhini / genetics*
Sperm Injections, Intracytoplasmic / veterinary

Substances

Endonucleases