The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Thomas J Gniadek; Michael T Forman; Isabella Martin; Ravit Arav-Boger; Alexandra Valsamakis

doi:10.1016/j.diagmicrobio.2017.05.013

The effect of a genetic variant on quantitative real-time PCR in a case of disseminated adenovirus infection

Diagn Microbiol Infect Dis. 2017 Sep;89(1):40-43. doi: 10.1016/j.diagmicrobio.2017.05.013. Epub 2017 Jun 1.

Authors

Thomas J Gniadek¹, Michael T Forman¹, Isabella Martin¹, Ravit Arav-Boger², Alexandra Valsamakis³

Affiliations

¹ Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD.
² Pediatric Infectious Disease, Johns Hopkins School of Medicine, Baltimore, MD.
³ Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD. Electronic address: avalsam1@jhmi.edu.

PMID: 28629877
DOI: 10.1016/j.diagmicrobio.2017.05.013

Abstract

A patient developed disseminated adenovirus infection following bone marrow transplant. TaqMan real-time PCR showed reduced maximum fluorescence in the amplification curve from all plasma samples. Sequencing revealed three single nucleotide mismatches between the TaqMan probe and probe binding region. Real-time PCR with probe matching the isolate sequence showed normal amplification and a higher copy number result.

Keywords: Aberrant real-time PCR; Adenovirus; Reduced fluorescence.

Publication types

Case Reports

MeSH terms

Adenovirus Infections, Human / diagnosis*
Adenovirus Infections, Human / virology
Adenoviruses, Human / genetics*
Adenoviruses, Human / isolation & purification*
Adolescent
Base Pair Mismatch
Bone Marrow Transplantation / adverse effects
DNA Primers / genetics
DNA, Viral / chemistry
DNA, Viral / genetics
Female
Genetic Variation*
Humans
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Analysis, DNA
Viremia / diagnosis*
Viremia / virology

Substances

DNA Primers
DNA, Viral