Recovery evaluation of rats' damaged tibias: Implantation of core-shell structured bone scaffolds made using hollow braids and a freeze-thawing process

Jia-Horng Lin; Mong-Chuan Lee; Chih-Kuang Chen; Chien-Lin Huang; Yueh-Sheng Chen; Shih-Peng Wen; Shu-Ting Kuo; Ching-Wen Lou

doi:10.1016/j.msec.2017.04.156

Recovery evaluation of rats' damaged tibias: Implantation of core-shell structured bone scaffolds made using hollow braids and a freeze-thawing process

Mater Sci Eng C Mater Biol Appl. 2017 Oct 1:79:481-490. doi: 10.1016/j.msec.2017.04.156. Epub 2017 May 5.

Authors

Jia-Horng Lin¹, Mong-Chuan Lee², Chih-Kuang Chen³, Chien-Lin Huang⁴, Yueh-Sheng Chen⁵, Shih-Peng Wen⁶, Shu-Ting Kuo⁶, Ching-Wen Lou⁷

Affiliations

¹ Innovation Platform of Intelligent and Energy-Saving Textiles, Tianjin Polytechnic University, Tianjin 300387, China; Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan; Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou 350108, China; School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Fashion Design, Asia University, Taichung 41354, Taiwan.
² Graduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.
³ The Polymeric Biomaterials Lab, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan.
⁴ Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan.
⁵ Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40402, Taiwan.
⁶ Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan.
⁷ Department of Chemistry and Chemical Engineering, Minjiang University, Fuzhou 350108, China; Graduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan; School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China. Electronic address: cwlou@ctust.edu.tw.

PMID: 28629044
DOI: 10.1016/j.msec.2017.04.156

Abstract

This study prepares biodegradable bone scaffolds helping the recovery of damaged tibias of rats. Polyvinyl alcohol (PVA) plied yarns are fabricated into hollow braids. The braids are combined with hydroxyapatite (HA)/gelatin/PVA mixtures and processed using freeze-thawing and freeze-drying processes in order to form bone scaffolds. These bone scaffolds are observed by scanning electron scope (SEM) and tested for compression strength. Afterwards, recovery of damaged bone, the morphology of the bone, and the histological observation are evaluated. Results indicate a small amount of HA helps in enhancing the compressive strength of bone scaffolds. Results of in vivo assay indicate the damaged tibias of rats recover and function well eight weeks after the implantation, and exhibit a normal morphology. Histological observation confirms the bone scaffolds gradually decompose, allowing tissue infiltration and facilitating ossification. This study successfully produces bone scaffolds with satisfactory mechanical properties helping in the recovery of damaged tibias of rats.

Keywords: Bone scaffolds; Braid; Hydroxyapatite (HA); Polyvinyl alcohol (PVA); Recovery of damaged bone.

MeSH terms

Animals
Bone and Bones
Durapatite
Freezing
Porosity
Rats
Tibia*
Tissue Engineering
Tissue Scaffolds

Substances

Durapatite