Multi-walled carbon nanotubes/graphene oxide hybrid and nanohydroxyapatite composite: A novel coating to prevent dentin erosion

Sídnei Nahorny; Hudson Zanin; Vinie Abreu Christino; Fernanda Roberta Marciano; Anderson Oliveira Lobo; Luís Eduardo Silva Soares

doi:10.1016/j.msec.2017.05.022

Multi-walled carbon nanotubes/graphene oxide hybrid and nanohydroxyapatite composite: A novel coating to prevent dentin erosion

Mater Sci Eng C Mater Biol Appl. 2017 Oct 1:79:199-208. doi: 10.1016/j.msec.2017.05.022. Epub 2017 May 5.

Authors

Sídnei Nahorny¹, Hudson Zanin², Vinie Abreu Christino³, Fernanda Roberta Marciano⁴, Anderson Oliveira Lobo⁵, Luís Eduardo Silva Soares⁶

Affiliations

¹ Laboratory of Biomedical Vibrational Spectroscopy, University of Vale do Paraiba, São José dos Campos, São Paulo CEP 12244-000, Brazil.
² Carbon Sci-Tech Labs, University of Campinas, Campinas, São Paulo CEP 13083-852, Brazil.
³ Laboratory of Biomedical Nanotechnology, University of Vale do Paraiba, São José dos Campos, São Paulo, CEP 12244-000, Brazil.
⁴ Laboratory of Biomedical Nanotechnology, University of Vale do Paraiba, São José dos Campos, São Paulo, CEP 12244-000, Brazil; Laboratory of Biomedical Nanotechnology, Universidade Brasil, Rua Carolina Fonseca 235, CEP 08230-030, Itaquera, São Paulo, Brazil; Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
⁵ Laboratory of Biomedical Nanotechnology, University of Vale do Paraiba, São José dos Campos, São Paulo, CEP 12244-000, Brazil; Laboratory of Biomedical Nanotechnology, Universidade Brasil, Rua Carolina Fonseca 235, CEP 08230-030, Itaquera, São Paulo, Brazil; Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; Pós Graduação em Ciências dos Materiais, Centro de Tecnologia, Universidade Federal do Piauí, 64049-550, Teresina, PI, Brazil. Electronic address: aolobo@pq.cnpq.br.
⁶ Laboratory of Biomedical Vibrational Spectroscopy, University of Vale do Paraiba, São José dos Campos, São Paulo CEP 12244-000, Brazil. Electronic address: lesoares@univap.br.

PMID: 28629008
DOI: 10.1016/j.msec.2017.05.022

Abstract

To date is emergent the development of novel coatings to protect erosion, especially to preventive dentistry and restorative dentistry. Here, for the first time we report the effectiveness of multi-walled carbon nanotube/graphene oxide hybrid carbon-base material (MWCNTO-GO) combined with nanohydroxyapatite (nHAp) as a protective coating for dentin erosion. Fourier transform Raman spectroscopy (FT-Raman), scanning electron (SEM), and transmission electron (TEM) microscopy were used to investigated the coatings and the effect of acidulated phosphate fluoride gel (APF) treatment on bovine teeth root dentin before and after erosion. The electrochemical corrosion performance of the coating was evaluated. Raman spectra identified that: (i) the phosphate (ν₁PO₄^3-) content of dentin was not significantly affected by the treatments and (ii) the carbonate (ν₁CO₃^2-) content in dentin increased when nHAp was used. However, the nHAp/MWCNTO-GO composite exposited lower levels of organic matrix (CH bonds) after erosion compared to other treatments. Interesting, SEM micrographs identified that the nHAp/MWCNTO-GO formed layers after erosive cycling when associate with APF treatment, indicating a possible chemical bond among them. Treatments of root dentin with nHAp, MWCNTO-GO, APF_MWCNTO-GO, and APF_nHAp/MWCNTO-GO increased the carbonate content, carbonate/phosphate ratio, and organic matrix band area after erosion. The potentiodynamic polarization curves and Nyquist plot showed that nHAp, MWCNT-GO and nHAp/MWCNT-GO composites acted as protective agents against corrosion process. Clearly, the nHAp/MWCNTO-GO composite was stable after erosive cycling and a thin and acid-resistant film was formed when associated to APF treatment.

Keywords: Carbon nanotubes oxide; Dentin erosion; FT-Raman spectroscopy; Graphene oxide; Nanohydroxyapatite.

MeSH terms

Animals
Carbonates
Cattle
Dentin
Graphite
Nanotubes, Carbon*
Oxides

Substances

Carbonates
Nanotubes, Carbon
Oxides
Graphite