Evaluation of the InnoTyper® 21 genotyping kit in multi-ethnic populations

Peter Gustav Ristow; Nicole Barnes; Gina Pineda Murphy; Hiromi Brown; Kevin Wesley Cloete; Maria Eugenia D'Amato

doi:10.1016/j.fsigen.2017.06.002

Evaluation of the InnoTyper^® 21 genotyping kit in multi-ethnic populations

Forensic Sci Int Genet. 2017 Sep:30:43-50. doi: 10.1016/j.fsigen.2017.06.002. Epub 2017 Jun 7.

Authors

Peter Gustav Ristow¹, Nicole Barnes², Gina Pineda Murphy³, Hiromi Brown³, Kevin Wesley Cloete², Maria Eugenia D'Amato⁴

Affiliations

¹ Forensic DNA Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, 7535, South Africa; Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark.
² Forensic DNA Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, 7535, South Africa.
³ InnoGenomics Technologies, 1441 Canal Street, Suite 307, New Orleans, LA, 70112, United States of America.
⁴ Forensic DNA Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, 7535, South Africa. Electronic address: medamato@uwc.com.

PMID: 28628900
DOI: 10.1016/j.fsigen.2017.06.002

Abstract

We report the findings of the evaluation of the InnoTyper^® 21 genotyping kit for the use of human identification (HID) and paternity testing in South Africa. This novel forensic kit evaluates 20 retrotransposable elements (AC4027, MLS26, ALU79712, NBC216, NBC106, RG148, NBC13, AC2265, MLS09, AC1141, TARBP, AC2305, HS4.69, NBC51, ACA1766, NBC120, NBC10, NBC102, SB19.12 and NBC148) and the Amelogenin locus for sex determination. The evaluation of the genotyping performance showed no significant spectral pull-up for peak heights between 100 and 30,000 RFUs. All loci presented biallelic patterns except the triallelic RG148 locus resulting from a variant insertion allele, named RG148I-1, observed exclusively in the Bantu. The InnoTyper^® 21 kit was found to be highly discriminatory between the 507 unrelated individuals of the Afrikaaner, Asian Indian, Coloured, amaXhosa and amaZulu groups. The HID parameters: the CPD ranged between 0.99999987 and 0.9999999845, and the CMP between 1.0335×10^-7 and 1.5506×10^-8. The paternity parameters: the CPI ranged between 0.0202 and 0.3177, and the CPE between 0.9161 and 0.9749. There were no significant signs of deviations from HWE or linkage disequilibrium (LD) after applying a Bonferroni correction. This kit also showed minor levels of population structure which could differentiate between the African and non-African population groups. Finally, in challenging casework with severely degraded biological material, the InnoTyper^® 21 genotyping kit was compatible with GlobalFiler^® and Investigator DIPplex^® to increase the HID parameters.

Keywords: Africa; Bantu; Binary markers; INNULS; InnoTyper.

Publication types

Evaluation Study

MeSH terms

Alleles
Amelogenin / genetics
DNA Fingerprinting / instrumentation*
Discriminant Analysis
Ethnicity / genetics*
Genetics, Population*
Genotype*
Humans
Microsatellite Repeats
Sex Determination Analysis

Substances

Amelogenin