A heme-dependent enzyme forms the nitrogen-nitrogen bond in piperazate

Yi-Ling Du; Hai-Yan He; Melanie A Higgins; Katherine S Ryan

doi:10.1038/nchembio.2411

A heme-dependent enzyme forms the nitrogen-nitrogen bond in piperazate

Nat Chem Biol. 2017 Aug;13(8):836-838. doi: 10.1038/nchembio.2411. Epub 2017 Jun 19.

Authors

Yi-Ling Du^{1

2}, Hai-Yan He², Melanie A Higgins², Katherine S Ryan²

Affiliations

¹ Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
² Department of Chemistry, The University of British Columbia, Vancouver, BC, Canada.

PMID: 28628093
DOI: 10.1038/nchembio.2411

Abstract

Molecules containing a nitrogen-nitrogen (N-N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N-N bond formation in an organic molecule. Here we report that the heme-dependent enzyme KtzT from Kutzneria sp. 744 catalyzes N-N bond formation in the biosynthesis of piperazate, a building block for nonribosomal peptides.

MeSH terms

Actinomycetales / enzymology
Mixed Function Oxygenases / chemistry
Mixed Function Oxygenases / metabolism*
Molecular Conformation
Nitrogen / chemistry
Nitrogen / metabolism*
Pyridazines / chemistry
Pyridazines / metabolism*

Substances

Pyridazines
piperazic acid
Mixed Function Oxygenases
Nitrogen

Associated data

PubChem-Substance/336851435
PubChem-Substance/336851436
PubChem-Substance/336851437
PubChem-Substance/336851438
PubChem-Substance/336851439
PubChem-Substance/336851440
PubChem-Substance/336851441