Relative examination of antioxidative enzymatic activities in plantlets of Cardiospermum halicacabum L. differentiated from hypocotyls in in vivo and ex vitro environment

Anushi A Jahan; Mohammad Anis; Ibrahim M Aref

doi:10.1016/j.btre.2014.08.004

Relative examination of antioxidative enzymatic activities in plantlets of Cardiospermum halicacabum L. differentiated from hypocotyls in in vivo and ex vitro environment

Biotechnol Rep (Amst). 2014 Aug 19:4:66-72. doi: 10.1016/j.btre.2014.08.004. eCollection 2014 Dec.

Authors

Anushi A Jahan¹, Mohammad Anis^{1

2}, Ibrahim M Aref²

Affiliations

¹ Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202002, India.
² Department of Plant Production, College of Food & Agricultural Sciences, King Saud University, PO Box 2460, Riyadh 11451, Saudi Arabia.

Abstract

A plant regeneration protocol was devised for Cardiospermum halicacabum by means of aseptically extracted 7 days old hypocotyls forming adventitious shoots on Murashige and Skoog (MS) medium harmonized with 0.7 μM thidiazuron (TDZ) producing a maximum of 18.20 ± 0.98 number of shoots in 94% cultures following 4 weeks. Subsequent subculturing for five passages, on a medium without plant growth regulators, tempted the highest shoot number (40.00 ± 1.15) with an average shoot length of 6.53 ± 0.49 cm after the fourth subculture. Histological sections confirmed the formation of multiple buds from hypocotyl explants. The expression of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase was found to be higher in acclimatized plants than in the in vitro cultured ones suggesting the involvement of these enzymes in shoot differentiation and in growth under external environment partly due to their ability to cope up with oxidative stress.

Keywords: APX, ascorbate peroxidase; Acclimatization; CAT, catalase; Cardiospermum halicacabum; GR, glutathione reductase; Hypocotyl; Micropropagation; ROS, reactive oxygen species; SOD, superoxide dismutase; Sapindaceae; TDZ, thidiazuron.