Transcriptome of barley under three different heavy metal stress reaction

Martina Kintlová; Nicolas Blavet; Radim Cegan; Roman Hobza

doi:10.1016/j.gdata.2017.05.016

Transcriptome of barley under three different heavy metal stress reaction

Genom Data. 2017 May 22:13:15-17. doi: 10.1016/j.gdata.2017.05.016. eCollection 2017 Sep.

Authors

Martina Kintlová¹, Nicolas Blavet¹, Radim Cegan², Roman Hobza^{1

2}

Affiliations

¹ Institute of Experimental Botany AS CR, Centre of the Region Hana for Biotechnological and Agricultural Research, Slechtitelu 31, CZ 78371 Olomouc, Holice, Czech Republic.
² Department of Plant Developmental Genetics, Institute of Biophysics of the CAS, v.v.i., Kralovopolska 135, CZ 61200 Brno, Czech Republic.

Abstract

In the present study, we used Illumina sequencing technology (HiSeq 2000) to sequence the transcriptome of barley (Hordeum vulgare L., cv. Morex) under three different heavy metal stress conditions: copper, zinc and cadmium. For each of those metals, the concentration causing a 50% inhibitory effect for root growth (EC₅₀) was determined. We sequenced the total RNA of both roots and shoots from barley with and without heavy metal treatments in three replicates. Raw reads of the transcriptome project have been deposited in NCBI's BioProject accession number PRJNA382490. The obtained transcriptomic data will be useful for further studies focusing on heavy metal tolerance and comparative transcriptome analysis in barley.

Keywords: Barley; Cadmium; Copper; Heavy metal; RNA-Seq; Transcriptome; Zinc.