Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae

Alex Prima; Kiyotaka Y Hara; Apridah Cameliawati Djohan; Norimasa Kashiwagi; Prihardi Kahar; Jun Ishii; Hideki Nakayama; Fumiyoshi Okazaki; Bambang Prasetya; Akihiko Kondo; Yopi; Chiaki Ogino

doi:10.1016/j.biortech.2017.05.190

Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae

Bioresour Technol. 2017 Dec;245(Pt B):1400-1406. doi: 10.1016/j.biortech.2017.05.190. Epub 2017 Jun 1.

Authors

Affiliations

¹ Research Center for Biotechnology, LIPI, Cibinong 16911, West Java, Indonesia.
² Department of Environmental and Life Sciences, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan; Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
³ Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
⁴ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
⁵ Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Division of Environmental Protection Technology, Institute of Environmental Science, Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
⁶ Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577, Kurimamachiya, Tsu, Mie 514-8507, Japan.
⁷ Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro, Tsurumi, Yokohama 230-0045, Japan.
⁸ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.

PMID: 28624243
DOI: 10.1016/j.biortech.2017.05.190

Abstract

This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, β-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources.

Keywords: Cell factory; Glutathione; Mannan-based bioresource; Mannanase/mannosidase-expressing yeast; Synthetic biology.

MeSH terms

Glutathione
Mannans*
Saccharomyces cerevisiae
beta-Mannosidase

Substances

Mannans
beta-1,4-mannan
beta-Mannosidase
Glutathione