Mechanical characterization via nanoindentation of the woven bone developed during bone transport

J Mora-Macías; A Pajares; P Miranda; J Domínguez; E Reina-Romo

doi:10.1016/j.jmbbm.2017.05.031

Mechanical characterization via nanoindentation of the woven bone developed during bone transport

J Mech Behav Biomed Mater. 2017 Oct:74:236-244. doi: 10.1016/j.jmbbm.2017.05.031. Epub 2017 Jun 1.

Authors

J Mora-Macías¹, A Pajares², P Miranda³, J Domínguez⁴, E Reina-Romo⁵

Affiliations

¹ Department of Mining, Mechanical, Energy and Building Engineering, University of Huelva, 21071 Palos de la Frontera, Huelva, Spain. Electronic address: juan.mora@dimme.uhu.es.
² Department of Mechanical, Energy and Materials Engineering, University of Extremadura, 06006 Badajoz, Spain. Electronic address: apajares@unex.es.
³ Department of Mechanical, Energy and Materials Engineering, University of Extremadura, 06006 Badajoz, Spain. Electronic address: pmiranda@unex.es.
⁴ Department of Mechanical Engineering and Manufacturing, University of Seville, 41092 Seville, Spain. Electronic address: jaime@us.es.
⁵ Department of Mechanical Engineering and Manufacturing, University of Seville, 41092 Seville, Spain. Electronic address: erreina@us.es.

PMID: 28623826
DOI: 10.1016/j.jmbbm.2017.05.031

Abstract

Nanoindentation has been used successfully in the determination of the mechanical properties of bone. Its application in fracture healing provides information on the evolution of material properties of the woven bone during regeneration process. However, this technique has not been applied in assessing the mechanical properties of woven bone during distraction osteogenesis. Therefore, the aim of this work is to evaluate the spatial and temporal variations of the elastic modulus of the woven bone generated during the bone transport process. Callus samples were harvested from intervened animals at different time points during the bone transport process (35, 50, 79, 98, 161 and 525 days after surgery) for nanoindentation measurements. Results clearly showed that the mean elastic modulus of the woven bone increased during the bone transport process reaching 77% of value for cortical bone after 525 days (from 7GPa 35 days after surgery to 14GPa 525 days after surgery approximately). Woven bone generated during bone transport seems to present similar evolution of elastic modulus with time as values reported for fracture healing. Furthermore, different spatial variations of elastic modulus within the callus were found for different stages of the process.

Keywords: Bone transport; Callus mechanical properties; Distraction osteogenesis; Nanoindentation; Woven bone.

MeSH terms

Animals
Bone Regeneration*
Bony Callus / physiology*
Elastic Modulus
Fracture Healing*
Osteogenesis, Distraction*
Sheep
Spatio-Temporal Analysis