Comment on "Xist recruits the X chromosome to the nuclear lamina to enable chromosome-wide silencing"

Chen-Yu Wang; John E Froberg; Roy Blum; Yesu Jeon; Jeannie T Lee

doi:10.1126/science.aal4976

Comment on "Xist recruits the X chromosome to the nuclear lamina to enable chromosome-wide silencing"

Science. 2017 Jun 16;356(6343):eaal4976. doi: 10.1126/science.aal4976.

Authors

Chen-Yu Wang¹, John E Froberg¹, Roy Blum¹, Yesu Jeon¹, Jeannie T Lee²

Affiliations

¹ Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, 185 Cambridge Street, Boston, MA 02114, USA.
² Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, 185 Cambridge Street, Boston, MA 02114, USA. lee@molbio.mgh.harvard.edu.

PMID: 28619887
DOI: 10.1126/science.aal4976

Abstract

Chen et al (Reports, 28 October 2016, p. 468) proposed that an interaction between Xist RNA and Lamin B receptor (LBR) is necessary and sufficient for Xist spreading during X-chromosome inactivation. We reanalyzed their data and found that reported genotypes of mutants are not supported by the sequencing data. These inconsistencies preclude assessment of the role of LBR in Xist spreading.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Comment

MeSH terms

Gene Silencing*
Nuclear Lamina*
RNA, Long Noncoding / genetics
RNA, Untranslated / genetics
X Chromosome
X Chromosome Inactivation

Substances

RNA, Long Noncoding
RNA, Untranslated

Grants and funding

R01 GM090278/GM/NIGMS NIH HHS/United States