Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse

Jerome Amoah; Naoya Ishizue; Miki Ishizaki; Misa Yasuda; Kenji Takahashi; Kazuaki Ninomiya; Ryosuke Yamada; Akihiko Kondo; Chiaki Ogino

doi:10.1016/j.biortech.2017.05.171

Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse

Bioresour Technol. 2017 Dec;245(Pt B):1413-1420. doi: 10.1016/j.biortech.2017.05.171. Epub 2017 May 31.

Authors

Jerome Amoah¹, Naoya Ishizue², Miki Ishizaki², Misa Yasuda², Kenji Takahashi³, Kazuaki Ninomiya⁴, Ryosuke Yamada⁵, Akihiko Kondo⁶, Chiaki Ogino⁷

Affiliations

¹ Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
² Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
³ Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
⁴ Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
⁵ Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai, Osaka 599-8531, Japan.
⁶ Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
⁷ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.

PMID: 28610972
DOI: 10.1016/j.biortech.2017.05.171

Abstract

This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (BGL, XYNII, EGII, CBHI and CBHII) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93g/l ethanol whiles that for LUKP produced 0.71g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass.

Keywords: Bioethanol; Cell surface display; Cocktail δ-integration; Enzyme activity; Lignocellulose.

MeSH terms

Biotechnology
Cellulose*
Ethanol*
Fermentation
Ionic Liquids

Substances

Ionic Liquids
Ethanol
Cellulose
bagasse