Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair

Methods Mol Biol. 2017:1640:145-158. doi: 10.1007/978-1-4939-7165-7_10.

Abstract

Plant ARGONAUTE (AGO) proteins regulate a wide range of cellular and developmental functions. Recent findings highlight their role during homologous recombination, a basic mechanism to repair double-strand DNA lesions (in somatic cells) and programmed DNA breaks (in meiocytes). This chapter contains an exhaustive description of procedures applied to analyze meiotic chromosome behavior (cytogenetic techniques) and DNA repair capacity (genotoxicity assays) in AGO-deficient Arabidopsis thaliana mutants.

Keywords: ARGONAUTEs; Arabidopsis; Chromosomes; DNA repair; FISH; Genotoxic agents; Homologous recombination; Meiosis; Small RNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Argonaute Proteins / genetics
  • Argonaute Proteins / metabolism*
  • Chromosomes, Plant / genetics
  • Cytogenetic Analysis / methods
  • DNA Damage
  • DNA Repair*
  • DNA, Plant / genetics
  • Gene Deletion
  • Homologous Recombination
  • In Situ Hybridization, Fluorescence / methods
  • Meiosis

Substances

  • Arabidopsis Proteins
  • Argonaute Proteins
  • DNA, Plant