Autoantibody responses to nodal and paranodal antigens in chronic inflammatory neuropathies

E K Mathey; N Garg; S B Park; T Nguyen; S Baker; N Yuki; C Yiannikas; C S Lin; J M Spies; R Ghaoui; M H Barnett; S Vucic; J D Pollard; M C Kiernan

doi:10.1016/j.jneuroim.2017.05.002

Autoantibody responses to nodal and paranodal antigens in chronic inflammatory neuropathies

J Neuroimmunol. 2017 Aug 15:309:41-46. doi: 10.1016/j.jneuroim.2017.05.002. Epub 2017 May 13.

Authors

E K Mathey¹, N Garg², S B Park², T Nguyen², S Baker³, N Yuki⁴, C Yiannikas⁵, C S Lin⁶, J M Spies⁷, R Ghaoui⁸, M H Barnett², S Vucic⁹, J D Pollard², M C Kiernan²

Affiliations

¹ Brain & Mind Centre, University of Sydney, Sydney, Australia. Electronic address: emily.mathey@sydney.edu.au.
² Brain & Mind Centre, University of Sydney, Sydney, Australia.
³ Department of Medicine, Physical Medicine & Neurology, Neuromuscular Disease Clinic, McMaster University, Hamilton, ON, Canada.
⁴ Department of Neurology, Mishima Hospital, Niigata, Japan.
⁵ Northern Clinical School, University of Sydney, Sydney, Australia.
⁶ School of Medical Sciences, Department of Physiology, Translational Neuroscience Facilities, University of New South Wales, New South Wales, Australia.
⁷ Institute of Clinical Neurosciences, Royal Prince Alfred Hospital, Sydney, Australia.
⁸ Neurology Department, Royal Adelaide Hospital, Adelaide, South Australia, Australia.
⁹ Western Clinical School, University of Sydney, New South Wales, Australia.

PMID: 28601286
DOI: 10.1016/j.jneuroim.2017.05.002

Abstract

Autoantibodies to nodal/paranodal proteins have been reported in patients with chronic inflammatory demyelinating polyneuropathy (CIDP) and multifocal motor neuropathy (MMN). To determine the frequency of anti-paranodal antibodies in our cohort of CIDP patients and to validate the presence anti-nodal antibodies in MMN, sera were screened for IgG against human neurofascin 155, contactin-1, neurofascin 186 and gliomedin using ELISA. In CIDP patients, 7% were anti-NF155 IgG₄ positive and 7% were anti-CNTN1 IgG₄ positive. Positive results were confirmed using cell based assays and indirect immunofluorescence on teased nerve fibres. We did not detect IgG autoantibodies against these nodal/paranodal antigens in MMN patients.

Keywords: Autoantibody; Chronic inflammatory demyelinating polyneuropathy; Contactin; Gliomedin; Multifocal motor neuropathy; Neurofascin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Animals
Autoantibodies / blood*
Autoantibodies / immunology
Cell Adhesion Molecules / blood
Cell Adhesion Molecules / immunology
Female
HeLa Cells
Humans
Immunoglobulin G / blood
Immunoglobulin G / immunology
Male
Membrane Proteins / blood
Membrane Proteins / immunology
Middle Aged
Nerve Growth Factors / blood
Nerve Growth Factors / immunology
Nerve Tissue Proteins / blood
Nerve Tissue Proteins / immunology
Polyneuropathies / blood*
Polyneuropathies / diagnosis*
Polyneuropathies / immunology
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / blood*
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / diagnosis*
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / immunology
Rats
Rats, Inbred Lew

Substances

Autoantibodies
Cell Adhesion Molecules
GLDN protein, human
Immunoglobulin G
Membrane Proteins
NFASC protein, human
Nerve Growth Factors
Nerve Tissue Proteins