Multilocus Sequence Typing (MLST) for Cronobacter spp

Susan Joseph; Stephen Forsythe

doi:10.1007/978-1-4939-7037-7_16

Multilocus Sequence Typing (MLST) for Cronobacter spp

Methods Mol Biol. 2017:1616:241-248. doi: 10.1007/978-1-4939-7037-7_16.

Authors

Susan Joseph¹, Stephen Forsythe²

Affiliations

¹ Institute of Dentistry, Barts and The London School of Medicine & Dentistry, Queen Mary University of London, Blizard Building, 4, Newark St., London, E1 2AT, UK.
² Pathogen Research Group, School of Science & Technology, Nottingham Trent University, Clifton Lane, Nottingham, UK. sforsythe4j@gmail.com.

PMID: 28600774
DOI: 10.1007/978-1-4939-7037-7_16

Abstract

MLST is a molecular typing technique that involves the identification and clustering of bacterial isolates based on the partial sequence analysis of multiple housekeeping genes (generally seven) which are distributed across the length of the genome of the organism. The Cronobacter whole genus MLST scheme can be successfully used for an accurate species level identification and classification of this complex genus.

Keywords: Clonal complex; Cronobacter; MLST; Neonatal meningitis; PubMLST; ST4; Sequence type.

MeSH terms

Alleles
Bacterial Typing Techniques / methods*
Cronobacter / classification*
Cronobacter / genetics*
Electrophoresis, Agar Gel / instrumentation
Electrophoresis, Agar Gel / methods
Multilocus Sequence Typing / methods*
Polymerase Chain Reaction / instrumentation
Polymerase Chain Reaction / methods*