Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

Silvia Fontenete; Marina Leite; Ceu Figueiredo; Paul Cos; Nuno F Azevedo

doi:10.1007/978-1-4939-7037-7_8

Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

Methods Mol Biol. 2017:1616:137-146. doi: 10.1007/978-1-4939-7037-7_8.

Authors

Silvia Fontenete^{1

2

3

4

5

6}, Marina Leite^{2

3}, Ceu Figueiredo^{2

3

7}, Paul Cos⁸, Nuno F Azevedo⁹

Affiliations

¹ LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
² i3s Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
³ IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal.
⁴ Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark.
⁵ ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal.
⁶ Epithelial Cell Biology Group, Cancer Cell Biology Program, Spanish National Cancer Research Centre, Madrid, 28029, Madrid, Spain.
⁷ FMUP, Faculty of Medicine of the University of Porto, Porto, Portugal.
⁸ Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
⁹ LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal. nazevedo@fe.up.pt.

PMID: 28600766
DOI: 10.1007/978-1-4939-7037-7_8

Abstract

In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only as a way to identify in vivo important microorganisms and their patterns of distribution within specific or at different anatomic sites, but also to better understand interaction mechanisms involving the microbiome and the human body.

Keywords: Diagnostics; FIVH; Fish; Helicobacter pylori; In vivo; Microbiology; Nucleic acids.

MeSH terms

Animals
DNA Probes
Disease Models, Animal
Gastric Mucosa / microbiology*
Helicobacter Infections / microbiology
Helicobacter pylori / genetics*
In Situ Hybridization, Fluorescence / methods*
Mice, Inbred C57BL
Microscopy, Fluorescence / methods

Substances

DNA Probes