Mycobacterial Load Assay

Stephen H Gillespie; Wilber Sabiiti; Katarina Oravcova

doi:10.1007/978-1-4939-7037-7_5

Mycobacterial Load Assay

Methods Mol Biol. 2017:1616:89-105. doi: 10.1007/978-1-4939-7037-7_5.

Authors

Stephen H Gillespie¹, Wilber Sabiiti², Katarina Oravcova²

Affiliations

¹ School of Medicine, University of St Andrews, North Haugh, North Street, St Andrews, KY16 9AJ, UK. shg3@st-andrews.ac.uk.
² School of Medicine, University of St Andrews, North Haugh, North Street, St Andrews, KY16 9AJ, UK.

PMID: 28600763
DOI: 10.1007/978-1-4939-7037-7_5

Abstract

Tuberculosis is a difficult disease to treat, a process made more harder as tools to monitor treatment response only provide a result long after the patient has provided a sample. The mycobacterial load assay (MBLA) provides a simple molecular test to quantify and determine the viability of M. tuberculosis in human or other samples.

Keywords: Mycobacterial load assay (MBLA); Reverse transcriptase real-time quantitative polymerase chain reaction (RT-qPCR); Ribosomal RNA (rRNA); Sputum.

MeSH terms

Bacterial Load / instrumentation
Bacterial Load / methods*
Bacterial Load / standards
Humans
Mycobacterium tuberculosis* / pathogenicity
Reverse Transcriptase Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / standards
Sensitivity and Specificity
Sputum / microbiology
Tuberculosis / microbiology*