Effects of CuO nanoparticles on Lemna minor

Guanling Song; Wenhua Hou; Yuan Gao; Yan Wang; Lin Lin; Zhiwei Zhang; Qiang Niu; Rulin Ma; Lati Mu; Haixia Wang

doi:10.1186/s40529-016-0118-x

Effects of CuO nanoparticles on Lemna minor

Bot Stud. 2016 Dec;57(1):3. doi: 10.1186/s40529-016-0118-x. Epub 2016 Jan 27.

Authors

Guanling Song^{1

2}, Wenhua Hou³, Yuan Gao⁴, Yan Wang⁵, Lin Lin¹, Zhiwei Zhang¹, Qiang Niu¹, Rulin Ma¹, Lati Mu¹, Haixia Wang¹

Affiliations

¹ School of Medicine, Shihezi University, Shihezi, 832000, China.
² Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Shanghai, 200433, China.
³ Research Center of Water Pollution Control Technology, Chinese Research Academy of Environment Sciences, Beijing, 100012, China.
⁴ Department of Life Science, Shandong University of Technology, Zibo, 255049, China.
⁵ School of Medicine, Shihezi University, Shihezi, 832000, China. keaiwangyan.student@sina.com.

Abstract

Background: Copper dioxide nanoparticles (NPs), which is a kind of important and widely used metal oxide NP, eventually reaches a water body through wastewater and urban runoff. Ecotoxicological studies of this kind of NPs effects on hydrophyte are very limited at present. Lemna minor was exposed to media with different concentrations of CuO NPs, bulk CuO, and two times concentration of Cu²⁺ released from CuO NPs in culture media. The changes in plant growth, chlorophyll content, antioxidant defense enzyme activities [i.e., peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) activities], and malondialdehyde (MDA) content were measured in the present study. The particle size of CuO NPs and the zeta potential of CuO NPs and bulk CuO in the culture media were also analyzed to complementally evaluate their toxicity on duckweed.

Result: Results showed that CuO NPs inhibited the plant growth at lower concentration than bulk CuO. L. minor roots were easily broken in CuO NPs media under the experimental condition, and the inhibition occurred only partly because CuO NPs released Cu²⁺ in the culture media. The POD, SOD, and CAT activities of L. minor increased when the plants were exposed to CuO NPs, bulk CuO NPs and two times the concentration of Cu²⁺ released from CuO NPs in culture media, but the increase of these enzymes were the highest in CuO NPs media among the three kinds of materials. The MDA content was significantly increased compared with that of the control from 50 mg L^-1 CuO NP concentration in culture media.

Conclusion: CuO NPs has more toxicity on L. minor compared with that of bulk CuO, and the inhibition occurred only partly because released Cu²⁺ in the culture media. The plant accumulated more reactive oxygen species in the CuO NP media than in the same concentration of bulk CuO. The plant cell encountered serious damage when the CuO NP concentration reached 50 mg L^-1 in culture media. The toxicology of CuO NP on hydrophytes must be considered because that hydrophytes are the basic of aquatic ecosystem.