Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Jeong Chan Joo; Young Hoon Oh; Ju Hyun Yu; Sung Min Hyun; Tae Uk Khang; Kyoung Hee Kang; Bong Keun Song; Kyungmoon Park; Min-Kyu Oh; Sang Yup Lee; Si Jae Park

doi:10.1016/j.biortech.2017.05.131

Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Bioresour Technol. 2017 Dec;245(Pt B):1692-1700. doi: 10.1016/j.biortech.2017.05.131. Epub 2017 May 21.

Authors

Affiliations

¹ Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 34602, Republic of Korea.
² Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 34602, Republic of Korea; Department of Biological and Chemical Engineering, Hongik University, 2639 Sejong-ro, Sinan-ri, Jochiwon-eup, Sejong-si 30016, Republic of Korea.
³ Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 34602, Republic of Korea; Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
⁴ Department of Biological and Chemical Engineering, Hongik University, 2639 Sejong-ro, Sinan-ri, Jochiwon-eup, Sejong-si 30016, Republic of Korea.
⁵ Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
⁶ Metabolic Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
⁷ Division of Chemical Engineering and Materials Science, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Republic of Korea. Electronic address: parksj93@ewha.ac.kr.

PMID: 28579174
DOI: 10.1016/j.biortech.2017.05.131

Abstract

This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation.

Keywords: 5-Aminovaleric acid; Corynebacterium glutamicum; Metabolic engineering; Miscanthus; Pretreatment.

MeSH terms

Amino Acids, Neutral*
Corynebacterium glutamicum*
Fermentation
Hydrolysis
Metabolic Engineering

Substances

Amino Acids, Neutral
5-aminovaleric acid