Starch-based nanocapsules fabricated through layer-by-layer assembly for oral delivery of protein to lower gastrointestinal tract

Yiping Zhang; Chengdeng Chi; Xiaoyi Huang; Qin Zou; Xiaoxi Li; Ling Chen

doi:10.1016/j.carbpol.2017.04.090

Starch-based nanocapsules fabricated through layer-by-layer assembly for oral delivery of protein to lower gastrointestinal tract

Carbohydr Polym. 2017 Sep 1:171:242-251. doi: 10.1016/j.carbpol.2017.04.090. Epub 2017 Apr 28.

Authors

Yiping Zhang¹, Chengdeng Chi¹, Xiaoyi Huang², Qin Zou¹, Xiaoxi Li³, Ling Chen⁴

Affiliations

¹ Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
² Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Department of Chemistry, Materials, and Chemical Engineering "G.Natta", Politecnico di Milano, Milan 20131, Italy.
³ Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: xxlee@scut.edu.cn.
⁴ Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: felchen@scut.edu.cn.

PMID: 28578960
DOI: 10.1016/j.carbpol.2017.04.090

Abstract

Anionic carboxymethyl starch (CMS) and cationic quaternary ammonium starch (QAS), were used to fabricate nanocapsules through electrostatic layer by layer (LbL) alternate deposition onto colloidal BSA particles. An ideal starch-based colloidal nanocapsule was achieved by adjusting the degree of substitution (DS) and weight average molecular molar mass (M_w) of CMS. The nanocapsules fabricated by CMS with lower DS or M_w possessed more compact and stable core-shell structure, which favoured the BSA delivery from the upper gastrointestinal tract (GIT) to the colon. In particular, CMS/QAS nanocapsules constructed by CMS with lower DS and M_w showed better colon-specific delivery and release performance in simulated GIT fluid after 7days' storage in different kinds of beverage (33.04%-46.35% in upper GIT, 52.70%-64.97% in colon, respectively). These findings demonstrated that CMS/QAS nanocapsules constructed by CMS with lower DS and M_w can be further exploited as a potential oral delivery system for protein to colon.

Keywords: Carboxymethyl starch; Colon targeting; Layer-by-layer assembly nanocapsules; Oral delivery; Protein; Quaternary ammonium starch.

MeSH terms

Administration, Oral
Colon*
Drug Delivery Systems
Molecular Weight
Nanocapsules / chemistry*
Proteins / administration & dosage*
Proteins / chemistry
Starch / chemistry*

Substances

Nanocapsules
Proteins
Starch