The suppression of innate immune response by human rhinovirus C

Li-Li Pang; Xin-Hui Yuan; Chang-Sheng Shao; Mao-Zhong Li; Ying Wang; Hui-Min Wang; Guang-Cheng Xie; Zhi-Ping Xie; Yue Yuan; Dong-Mei Zhou; Xiao-Man Sun; Qing Zhang; Yan Xin; Dan-di Li; Zhao-Jun Duan

doi:10.1016/j.bbrc.2017.05.169

The suppression of innate immune response by human rhinovirus C

Biochem Biophys Res Commun. 2017 Aug 12;490(1):22-28. doi: 10.1016/j.bbrc.2017.05.169. Epub 2017 May 31.

Authors

Li-Li Pang¹, Xin-Hui Yuan², Chang-Sheng Shao³, Mao-Zhong Li⁴, Ying Wang⁵, Hui-Min Wang⁵, Guang-Cheng Xie⁶, Zhi-Ping Xie¹, Yue Yuan⁵, Dong-Mei Zhou¹, Xiao-Man Sun¹, Qing Zhang¹, Yan Xin⁷, Dan-di Li⁸, Zhao-Jun Duan⁹

Affiliations

¹ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
² The First Hospital of Lanzhou University, Lanzhou 730000, China.
³ Anhui Engineering Research Center of Recombinant Protein Pharmaceutical Biotechnology, Institute of Advanced Technology, University of Science and Technology of China, Hefei 230026, China.
⁴ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Institute for Immunization and Prevention, Beijing CDC, Beijing 100013, China.
⁵ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Basic Medical College, Inner Mongolia Medical University, Hohhot 010000, China.
⁶ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Pathogenic Biology, Chengde Medical University, Chengde 067000, China.
⁷ Basic Medical College, Inner Mongolia Medical University, Hohhot 010000, China.
⁸ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address: dandili@126.com.
⁹ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. Electronic address: zhaojund@126.com.

PMID: 28576493
DOI: 10.1016/j.bbrc.2017.05.169

Abstract

Rhinovirus C (RV-C), a newly identified group of human rhinoviruses (RVs), is associated with exacerbation of severe asthma. The type I interferon (IFN) response induced by this virus and the mechanisms of evasion of IFN-mediated innate immunity for RV-C remain unclear. In this study, we constructed a full-length cDNA clone of RV-C (LZ651) from a clinical sample. IFN-β mRNA and protein levels were not elevated in differentiated Human bronchial epithelial (HBE) cells at the air-liquid interface infected with RV-C, except in the early stage of infection. The ability to attenuate IFN-β activation was ascribed to 3C^pro of RV-C, and the 40-His site of 3C^pro played an important role. Furthermore, RIG-I was degraded by 3C^pro in a caspase-dependent manner and 3C^pro cleaved MAVS at 148 Q/A, which inhibited IFN signaling. Taken together, our results demonstrate the mechanism by which RV-C circumvents the production of type I IFN in infected cells.

Keywords: 3C protein; Interferon response; MAVS; RIG-I signaling pathway; Rhinovirus C.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HEK293 Cells
HeLa Cells
Humans
Immune Tolerance*
Immunity, Innate / immunology*
Interferon Type I / immunology
Rhinovirus / immunology*

Substances

Interferon Type I