Systematic analysis of the G-box Factor 14-3-3 gene family and functional characterization of GF14a in Brachypodium distachyon

Li Yang; Jun You; Yanping Wang; Jinzhu Li; Wenli Quan; Mingzhu Yin; Qingfeng Wang; Zhulong Chan

doi:10.1016/j.plaphy.2017.05.013

Systematic analysis of the G-box Factor 14-3-3 gene family and functional characterization of GF14a in Brachypodium distachyon

Plant Physiol Biochem. 2017 Aug:117:1-11. doi: 10.1016/j.plaphy.2017.05.013. Epub 2017 May 25.

Authors

Li Yang¹, Jun You², Yanping Wang³, Jinzhu Li¹, Wenli Quan⁴, Mingzhu Yin¹, Qingfeng Wang⁵, Zhulong Chan⁶

Affiliations

¹ Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, Hubei, 430074, China; University of Chinese Academy of Sciences, Beijing 100039, China.
² Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei, 430062, China.
³ Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
⁴ Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan, Hubei, 432000, China.
⁵ Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden/Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, Hubei, 430074, China. Electronic address: qfwang@wbgcas.cn.
⁶ Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan, Hubei, 432000, China. Electronic address: zlchan@hzau.edu.cn.

PMID: 28575641
DOI: 10.1016/j.plaphy.2017.05.013

Abstract

The 14-3-3 proteins are highly conserved and ubiquitously found in eukaryotes. Plant 14-3-3 proteins are involved in many signaling pathways to regulate plant growth and development. Here we identified seven Brachypodium distachyon 14-3-3 genes and analyzed the evolution, gene structure and expression profiles of these genes. Several cis-elements involved in stress response and hormone pathway were found in the promoter region of 14-3-3 genes. Results of gene expression analysis showed that these genes were induced by abiotic stresses or hormone treatments. Transgenic Arabidopsis overexpressing BdGF14a exhibited increased leaf water content (LWC) and decreased electrolyte leakage (EL) and showed improved drought stress tolerance. BdGF14a transgene significantly up-regulated expression levels of DREB1A and DREB1B, but slightly elevated ABI1 expression. These results indicated that BdGF14a functioned as a positive regulator in plant response to drought stress mainly via ABA independent pathway.

Keywords: 14-3-3 protein; Abiotic stress; Antioxidant enzyme; BdGF14a; Brachypodium distachyon; DREB1.

MeSH terms

14-3-3 Proteins / genetics*
14-3-3 Proteins / metabolism*
Brachypodium / genetics*
Brachypodium / metabolism*
Brachypodium / physiology
Droughts*
Gene Expression Regulation, Plant
Plant Proteins / genetics*
Plant Proteins / metabolism*
Stress, Physiological / genetics

Substances

14-3-3 Proteins
Plant Proteins