Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells

Methods Mol Biol. 2017:1628:227-241. doi: 10.1007/978-1-4939-7116-9_18.

Abstract

In this chapter, we describe a Western blot assay for the successful detection of apoptosis in Ebola virus (EBOV)-infected cells. The protocol includes all steps from cell culture, infection of cells, generation of lysates, and analysis using Western blot to detect caspase cleavage as marker of apoptosis.

Keywords: Antibodies; Apoptosis; Caspases; Ebola virus; Filoviruses; Western blot; Zaire ebolavirus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis / genetics*
  • Blotting, Western / methods*
  • Caspases / genetics
  • Cell Count
  • Ebolavirus / genetics*
  • Ebolavirus / pathogenicity
  • Hemorrhagic Fever, Ebola / genetics*
  • Hemorrhagic Fever, Ebola / virology
  • Humans

Substances

  • Caspases