Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence

Methods Mol Biol. 2017:1628:211-225. doi: 10.1007/978-1-4939-7116-9_17.

Abstract

In this chapter, the use of immunofluorescence analysis as a tool to examine stress granule (SG) formation in Ebola virus (EBOV)-infected cells is described. The following protocol focuses on the process of inducing and analyzing the cellular stress response, including treatment of cells with inducers and inhibitors of the SG formation, and also describes EBOV infection, DNA transfection, and the usage of different cell lines.

Keywords: Antibodies; Cellular stress response; Ebola virus; Filoviruses; Immunofluorescence; Microscopy; Sodium arsenite; Stress granules; Zaire ebolavirus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies, Viral / isolation & purification
  • Cell Line
  • Ebolavirus / growth & development
  • Ebolavirus / metabolism*
  • Fluorescent Antibody Technique / methods*
  • Hemorrhagic Fever, Ebola / metabolism
  • Hemorrhagic Fever, Ebola / physiopathology
  • Hemorrhagic Fever, Ebola / virology
  • Humans
  • Stress, Physiological / genetics*

Substances

  • Antibodies, Viral